Identifying novel bioactive metabolites from human gut microbiota at Novo Nordisk Foundation Center for Basic Metabolic Research

Posted 1 week ago

This is a project proposal for a student opportunity at the Novo Nordisk Foundation Center for Basic Metabolic Research (CBMR).

Background: The human gastrointestinal tract harbors trillions of microorganisms referred to as the gut microbiota. Gut microbiota plays crucial role in the transformation of food into interesting bioactive metabolites (e.g., short chain fatty acids, lipids, vitamins, antimicrobials and neurotransmitters) that significantly impacts human health. However, many of these metabolites are yet to be identified and in-depth knowledge of these metabolites is still far to be fully elucidated. Hence, in order to identify novel metabolites, and its underlying complex network, untargeted metabolomic profiling of gut microbiome is the key.

The project: The aim of this project is to identify novel bioactive metabolites produced by the human gut microbiota. In order to realize this, we will grow gut microbiota in specially designed anaerobic fermenters and perform untargeted metabolomics and proteomics analysis using GC and LC-MS based methods. This project will provide training in culturing gut microbiota, performing cell count and qPCR, extraction of metabolites using various organic solvents, and performing various analytical techniques and data analysis.

The student: You are a team player, enthusiastic to learn about human gut microbiome and interested in working with microbiological and analytical techniques.

Contact: Please contact Associate professor Manimozhiyan Arumugam (arumugam@sund.ku.dk) or postdoc Thiyagarajan Gnanasekaran (thiyagarajan.gnanasekaran@sund.ku.dk) if you are interested or would like more information.

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